Background: FOP is an ultra-rare genetic disorder characterized by heterotopic ossification (HO) of soft and connective tissues (often preceded by flare-ups), cumulative disability, and shortened life expectancy. Palovarotene is a highly selective retinoic acid receptor-gamma agonist under investigation for treatment of FOP. Conducting FOP trials have multiple challenges, including low number of confirmed cases worldwide, limited understanding of disease progression, the need to identify disease-specific biomarkers and optimize assessment of HO progression.
Objective: To describe the challenges encountered when conducting clinical trials in FOP.
Methods: Here, we describe the methodology of: a non-interventional, prospective, protocol-specified, longitudinal natural history study (NHS; NCT02322255); a multicenter, randomized, double‑blind, placebo-controlled phase II trial (NCT02190747); an ongoing open-label extension (OLE) to the phase II trial (NCT02279095) (Table). Studies were designed adaptively. Palovarotene doses were administered episodically (high dose for 2 or 4-weeks, followed by low dose for 4 or ≥8-weeks, from flare-up onset) or daily. In the double-blind period of the phase II trial, participants were randomized in two cohorts: 0:3:1 (aged ≥15years) or 3:3:2 (≥6years) to palovarotene 5/2.5mg, palovarotene 10/5mg or placebo, episodically for 2-weeks then 4-weeks. HO incidence and volume are assessed annually by low dose whole-body computer tomography and/or at 12-weeks during the course of flare-ups (defined as ≥2 [≥1 in OLE Part C] or pain, swelling, stiffness, decreased range of motion, redness, or warmth). Other clinical, functional and patient-reported outcomes are assessed using FOP-specific measures, including the Cumulative Analogue Joint Involvement Scale (CAJIS) and FOP Physical Function Questionnaire (FOP-PFQ). Studies were approved by independent ethics committees. 151 unique participants were enrolled.
Conclusions: Novel methodological approaches are needed to develop disease-modifying treatments for serious, ultra-rare diseases. HO is the main cause of disability in patients with FOP. This program may inform development of new treatments in rare diseases.